Abstract: Large amount and wide distribution of wells containing hydrogen sulfide make it difficult to conduct sulfide treatment in Gudao. The high hydrogen sulfide wells mainly distribute in the steam flooding block. Single well wellhead casings were mainly used for the treatment of desulfurization agent and turned out it worked well. In recent years, with the development of extended oilfields, non-thermal production wells in some blocks of Kenxi Oilfield have also produced high-content hydrogen sulfide gas, which is widely distributed, using a large amount of chemicals, and causing the high cost of comprehensive treatment. At the same time, the mineralization of the produced fluid in the extended oilfields is high, and the compatibility of conventional desulfurizers is poor, which promotes scaling and affects production. Non-thermal production wells are not in a stable high temperature and high pressure environment, the cause of hydrogen sulfide generation is still unclear, and there is no good targeted treatment measures. Therefore, for the peripheral oilfield blocks of Gudao, in this paper, the mechanism of hydrogen sulfide generation in non-thermal production wells and the research on biological desulfurization technology in non-thermal production wells have been camied out, which obtained obvious application result and effectively solved the safety and environmental problems in the production site, it has good promotion prospects.