Abstract: with the rapid development of GPS technology, the accuracy of DGPS positioning becomes higher and higher, which makes it possible for precise calibration and verification of positioning auxiliary device in geophysical prospecting ship. Through calibration and verification of the auxiliary equipment of the Discoverer Six, a set of feasible operation method for calibration and verification of the auxiliary equipment have been developed. In this paper, calibration method and feasibility of calibration and verification have been described in detail, and error sources during calibration and verification are discussed in order to improve the calibration and verification accuracy of positioning auxiliary equipment.