Abstract: To study the relationship between Nickel and Vanadium distribution characteristics with HC accumulation, statistic analysis has been conducted on the content of Nickel and Vanadium on the basis of core, core borings and crude oil data from Junggar Basin. The study results showed that the content of Nickel and Vanadium is the largest in mudstones, secondarily in sandstones, and very small in oil samples and oil-bearing sands (bitumen). In stratigraphy, the content of Nickel and Vanadium is the largest in Jurassic and Permian. Statistic analysis has also been conducted on the planar distribution of Nickel and Vanadium in the target Jurassic. The statistic analyzing results on the content of Nickel and Vanadium showed that the southeast part of Block A is favorable area for HC exploration, with low value of Nickel and Vanadium contents. The south part of Block F is also the favorable area for HC exploration, with low value of Nickel and Vanadium contents. When conducting well production testing in individual well, the content of Nickel and Vanadium is large at oil-water layers or oil bearing water layers, and small in pure oil layers, which indicated that water layers will increase the content of Nickel and Vanadium, and oil layers will decrease the content of Nickel and Vanadium. When conducting well production testing in individual well, the ratio of Nickel and Vanadium is 1 at pure water layers. Finally, it is pointed out that from A to G Blocks, the areas with low content of Nickel and Vanadium are favorable HC exploration areas in Junggar Basin.